![](/images/graphics-bg.png)
Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood
المؤلفون المشاركون
Ayub, Seemi
Grégoire, Marie-Chantal
Kilpatrick, Michael W.
Krabchi, Kada
Samassékou, Oumar
Gadji, Macoura
Tafas, Triantafyllos
Lamoureux, Josée
Hemmings, Francis
Drouin, Régen
Ntwari, Aimé
Emad, Ahmed
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-08
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Fluorescence in situ hybridization (FISH) and manual scanning is a widely used strategy for retrieving rare cellular events such as fetal cells in maternal blood.
In order to determine the efficiency of these techniques in detection of rare cells, slides of XX cells with predefined numbers (1–10) of XY cells were prepared.
Following FISH hybridization, the slides were scanned blindly for the presence of XY cells by different observers.
The average detection efficiency was 84% (125/148).
Evaluation of probe hybridization in the missed events showed that 9% (2/23) were not hybridized, 17% (4/23) were poorly hybridized, while the hybridization was adequate for the remaining 74% (17/23).
In conclusion, manual scanning is a relatively efficient method to recover rare cellular events, but about 16% of the events are missed; therefore, the number of fetal cells per unit volume of maternal blood has probably been underestimated when using manual scanning.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Emad, Ahmed& Ayub, Seemi& Samassékou, Oumar& Grégoire, Marie-Chantal& Gadji, Macoura& Ntwari, Aimé…[et al.]. 2012. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484998
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Emad, Ahmed…[et al.]. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-484998
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Emad, Ahmed& Ayub, Seemi& Samassékou, Oumar& Grégoire, Marie-Chantal& Gadji, Macoura& Ntwari, Aimé…[et al.]. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484998
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-484998
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)