Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood
Joint Authors
Ayub, Seemi
Grégoire, Marie-Chantal
Kilpatrick, Michael W.
Krabchi, Kada
Samassékou, Oumar
Gadji, Macoura
Tafas, Triantafyllos
Lamoureux, Josée
Hemmings, Francis
Drouin, Régen
Ntwari, Aimé
Emad, Ahmed
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Fluorescence in situ hybridization (FISH) and manual scanning is a widely used strategy for retrieving rare cellular events such as fetal cells in maternal blood.
In order to determine the efficiency of these techniques in detection of rare cells, slides of XX cells with predefined numbers (1–10) of XY cells were prepared.
Following FISH hybridization, the slides were scanned blindly for the presence of XY cells by different observers.
The average detection efficiency was 84% (125/148).
Evaluation of probe hybridization in the missed events showed that 9% (2/23) were not hybridized, 17% (4/23) were poorly hybridized, while the hybridization was adequate for the remaining 74% (17/23).
In conclusion, manual scanning is a relatively efficient method to recover rare cellular events, but about 16% of the events are missed; therefore, the number of fetal cells per unit volume of maternal blood has probably been underestimated when using manual scanning.
American Psychological Association (APA)
Emad, Ahmed& Ayub, Seemi& Samassékou, Oumar& Grégoire, Marie-Chantal& Gadji, Macoura& Ntwari, Aimé…[et al.]. 2012. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484998
Modern Language Association (MLA)
Emad, Ahmed…[et al.]. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-484998
American Medical Association (AMA)
Emad, Ahmed& Ayub, Seemi& Samassékou, Oumar& Grégoire, Marie-Chantal& Gadji, Macoura& Ntwari, Aimé…[et al.]. Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization : Simulation of the Detection of Fetal Cells in Maternal Blood. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-484998
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-484998