Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis
المؤلفون المشاركون
Leggett, Robert E.
Fitzpatrick, Brittany
Schuler, Catherine
Levin, Robert M.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-29
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Purpose.
Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit.
Materials and Methods.
Rabbit bladder muscle and mucosa were obtained from control rabbits.
For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane.
The optical density was measured using a Kodak Scanner.
Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period.
Stop buffer was added to the plate and the results were quantified via the plate reader.
Results.
For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle.
However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis.
Discussion.
Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue.
This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fitzpatrick, Brittany& Schuler, Catherine& Leggett, Robert E.& Levin, Robert M.. 2012. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-485568
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fitzpatrick, Brittany…[et al.]. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-485568
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fitzpatrick, Brittany& Schuler, Catherine& Leggett, Robert E.& Levin, Robert M.. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-485568
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-485568
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر