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Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis
Joint Authors
Leggett, Robert E.
Fitzpatrick, Brittany
Schuler, Catherine
Levin, Robert M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Purpose.
Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit.
Materials and Methods.
Rabbit bladder muscle and mucosa were obtained from control rabbits.
For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane.
The optical density was measured using a Kodak Scanner.
Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period.
Stop buffer was added to the plate and the results were quantified via the plate reader.
Results.
For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle.
However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis.
Discussion.
Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue.
This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.
American Psychological Association (APA)
Fitzpatrick, Brittany& Schuler, Catherine& Leggett, Robert E.& Levin, Robert M.. 2012. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-485568
Modern Language Association (MLA)
Fitzpatrick, Brittany…[et al.]. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-485568
American Medical Association (AMA)
Fitzpatrick, Brittany& Schuler, Catherine& Leggett, Robert E.& Levin, Robert M.. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis. ISRN Urology. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-485568
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-485568