RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma

الملخص EN

A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma.

Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector.

The LOD and LOQ were 3.0 and 10.0 μg/L, respectively.

The method is linear in the interval 50.0–1000.0 μg/L.

The average extraction recovery of drug from plasma was found to be 92.2%.

The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%.

The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.