RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma
Joint Authors
Saboo, Shweta
Bankey, Shravan
Jain, Deepti
Khadabadi, S. S.
Lamale, Jasvant
Tapadiya, Ganesh
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-23
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
A simple, accurate, and precise HPLC method was developed and validated for determination of rimonabant in human plasma.
Following liquid-liquid extraction, chromatographic separation was accomplished using C18 column with mobile phase consisting of acetonitrile : water (90 : 10, v/v), drug was detected at 260 nm using UVdetector.
The LOD and LOQ were 3.0 and 10.0 μg/L, respectively.
The method is linear in the interval 50.0–1000.0 μg/L.
The average extraction recovery of drug from plasma was found to be 92.2%.
The percent CV of the method was found to be less than 10.8%, and accuracy was found between 94.5 and 106.7%.
The assay may be applied to a pharmacokinetic and bioequivalence study of rimonabant.
American Psychological Association (APA)
Bankey, Shravan& Tapadiya, Ganesh& Lamale, Jasvant& Jain, Deepti& Saboo, Shweta& Khadabadi, S. S.. 2012. RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-486184
Modern Language Association (MLA)
Bankey, Shravan…[et al.]. RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-486184
American Medical Association (AMA)
Bankey, Shravan& Tapadiya, Ganesh& Lamale, Jasvant& Jain, Deepti& Saboo, Shweta& Khadabadi, S. S.. RP-HPLC Method Development and Its Validation for Quantitative Determination of Rimonabant in Human Plasma. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-486184
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486184
