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The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
المؤلفون المشاركون
Kodera, Yoshio
Yoshitomi, Hideyuki
Obul, Jurat
Matsushita, Kazuyuki
Sogawa, Kazuyuki
Takano, Shigetsugu
Abulaizi, Mayinuer
Satoh, Mamoru
Nomura, Fumio
Miyazaki, Masaru
Tomonaga, Takeshi
المصدر
International Journal of Proteomics
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-10-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis.
Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography.
Proteins in each fraction were separated by two-dimensional gel electrophoresis.
Then the gel was stained by Coomassie Brilliant Blue.
Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS.
One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry.
These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA).
As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abulaizi, Mayinuer& Tomonaga, Takeshi& Satoh, Mamoru& Sogawa, Kazuyuki& Matsushita, Kazuyuki& Kodera, Yoshio…[et al.]. 2011. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-486427
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abulaizi, Mayinuer…[et al.]. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-486427
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abulaizi, Mayinuer& Tomonaga, Takeshi& Satoh, Mamoru& Sogawa, Kazuyuki& Matsushita, Kazuyuki& Kodera, Yoshio…[et al.]. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-486427
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-486427
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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