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The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer
Joint Authors
Kodera, Yoshio
Yoshitomi, Hideyuki
Obul, Jurat
Matsushita, Kazuyuki
Sogawa, Kazuyuki
Takano, Shigetsugu
Abulaizi, Mayinuer
Satoh, Mamoru
Nomura, Fumio
Miyazaki, Masaru
Tomonaga, Takeshi
Source
International Journal of Proteomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
We searched for novel tumor markers of pancreatic cancer by three-step serum proteome analysis.
Twelve serum abundant proteins were depleted using immunoaffinity columns followed by fractionation by reverse-phase high-performance liquid chromatography.
Proteins in each fraction were separated by two-dimensional gel electrophoresis.
Then the gel was stained by Coomassie Brilliant Blue.
Protein spots in which the expression levels were significantly different between cancer and normal control were identified by LC-MS/MS.
One hundred and two spots were upregulated, and 84 spots were downregulated in serum samples obtained from patients with pancreatic cancers, and 58 proteins were identified by mass spectrometry.
These candidate proteins were validated using western blot analysis and enzyme-linked immunosorbent assay (ELISA).
As a result of these validation process, we could confirm that the serum levels of apolipoprotein A-IV, vitamin D-binding protein, plasma retinol-binding protein 4, and tetranectin were significantly decreased in patients with pancreatic cancer.
American Psychological Association (APA)
Abulaizi, Mayinuer& Tomonaga, Takeshi& Satoh, Mamoru& Sogawa, Kazuyuki& Matsushita, Kazuyuki& Kodera, Yoshio…[et al.]. 2011. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-486427
Modern Language Association (MLA)
Abulaizi, Mayinuer…[et al.]. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-486427
American Medical Association (AMA)
Abulaizi, Mayinuer& Tomonaga, Takeshi& Satoh, Mamoru& Sogawa, Kazuyuki& Matsushita, Kazuyuki& Kodera, Yoshio…[et al.]. The Application of a Three-Step Proteome Analysis for Identification of New Biomarkers of Pancreatic Cancer. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-486427
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-486427