Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

المؤلفون المشاركون

Mirzaei, Maryam
Elias, Cynthia B.
Prakash, Satya
Xu, Yan

المصدر

Journal of Biomedicine and Biotechnology

العدد

المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2008-10-15

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Human interleukin-7 (hIL-7) is a cytokine secreted by the stromal cells of the red marrow.

It is important for proliferation during certain stages of B-cell maturation and for T and NK cell survival, development, and homeostasis.

It is a critical growth factor for enhancement and recovery of the immune T-cell.

Because of its strong immunomodulatory effects, hIL-7 may become a valuable supplementary agent for immunotherapeutical treatments in patients with HIV infection or immunodeficiency.

Human IL-7 has previously been produced in various protein expression systems.

In this paper, we present an alternative expression system, in Spodoptera frugiperda cells, for the production of hIL-7 using nonlytic vector systems.

This system allows generation of correctly translated and accurately processed heterologous proteins as soluble recombinant proteins.

Here we report plasmid construction, transfection, and consequent expression of hIL-7 using this nonlytic insect cell expression system.

The levels of secreted hIL-7 in a small scale experiment reached a level of 1.7 μg·1−1 under serum-free cell culture conditions.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Mirzaei, Maryam& Xu, Yan& Elias, Cynthia B.& Prakash, Satya. 2008. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487175

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Mirzaei, Maryam…[et al.]. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-487175

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Mirzaei, Maryam& Xu, Yan& Elias, Cynthia B.& Prakash, Satya. Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein. Journal of Biomedicine and Biotechnology. 2008. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-487175

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-487175