Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector

الملخص EN

A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed.

Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positive ζ potential (42.47 mV) and buffering effect.

These nanoparticles with N/P ratio of 30 give high transfection efficiency 2.12×104 RLU/μg protein and cell viability (86.4%).

These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy.