Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells

المؤلفون المشاركون

Perelman, Juliy M.
Wang, Xiaolong
Kolosov, Victor P.
Li, Qi
Zhou, Xiangdong

المصدر

Journal of Biomedicine and Biotechnology

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-02-14

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الطب البشري

الملخص EN

Mucus hypersecretion is a distinguished feature of chronic inflammatory airway diseases.

Interestingly, in this condition thyroid function is impaired with decreased level of triiodothyronine (T3), indicating potential link between low level of T3 and mucus hypersecretion.

But the underlying mechanisms are poorly understood.

In this study we aimed to elucidate the effect of T3 on MUC5AC secretion in human bronchial epithelial HBE16 cells and further investigate how T3 regulates MUC5AC gene expression at transcriptional level.

By RT-PCR and ELISA we showed that T3 inhibited MUC5AC mRNA expression and protein secretion in HBE16 cells.

Furthermore, luciferase assay and site-directed mutagenesis analysis demonstrated that T3 repressed MUC5AC expression at transcriptional level and the mechanism might partly lie in the specific inhibition of Sp1 binding to the promoter.

Our results suggest that decreased T3 level leads to the release of repression of MUC5AC expression and thus contributes to mucus hypersecretion.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Wang, Xiaolong& Li, Qi& Zhou, Xiangdong& Kolosov, Victor P.& Perelman, Juliy M.. 2012. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488007

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Wang, Xiaolong…[et al.]. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-488007

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Wang, Xiaolong& Li, Qi& Zhou, Xiangdong& Kolosov, Victor P.& Perelman, Juliy M.. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488007

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-488007