Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells
Joint Authors
Perelman, Juliy M.
Wang, Xiaolong
Kolosov, Victor P.
Li, Qi
Zhou, Xiangdong
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Mucus hypersecretion is a distinguished feature of chronic inflammatory airway diseases.
Interestingly, in this condition thyroid function is impaired with decreased level of triiodothyronine (T3), indicating potential link between low level of T3 and mucus hypersecretion.
But the underlying mechanisms are poorly understood.
In this study we aimed to elucidate the effect of T3 on MUC5AC secretion in human bronchial epithelial HBE16 cells and further investigate how T3 regulates MUC5AC gene expression at transcriptional level.
By RT-PCR and ELISA we showed that T3 inhibited MUC5AC mRNA expression and protein secretion in HBE16 cells.
Furthermore, luciferase assay and site-directed mutagenesis analysis demonstrated that T3 repressed MUC5AC expression at transcriptional level and the mechanism might partly lie in the specific inhibition of Sp1 binding to the promoter.
Our results suggest that decreased T3 level leads to the release of repression of MUC5AC expression and thus contributes to mucus hypersecretion.
American Psychological Association (APA)
Wang, Xiaolong& Li, Qi& Zhou, Xiangdong& Kolosov, Victor P.& Perelman, Juliy M.. 2012. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488007
Modern Language Association (MLA)
Wang, Xiaolong…[et al.]. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-488007
American Medical Association (AMA)
Wang, Xiaolong& Li, Qi& Zhou, Xiangdong& Kolosov, Victor P.& Perelman, Juliy M.. Triiodothyronine Represses MUC5AC Expression by Antagonizing Sp1 Binding to Its Promoter in Human Bronchial Epithelial HBE16 Cells. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-488007
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-488007