Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration
المؤلفون المشاركون
Borger, Pieter
Zhong, Jun
Tamm, Michael
Miglino, Nicola
Keglowich, Laura
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-30
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Bronchial smooth muscle cells (BSMC) are a major source of proinflammatory and proangiogenic cytokines and chemokines, including VEGF and CXC-chemokines.
CXC-chemokines act primarily on neutrophils, mediating their recruitment to and activation at the site of inflammation.
In humans, house-dust mite (HDM) allergens can cause asthmatic exacerbations and trigger an inflammatory response through protease-dependent mechanisms.
Objective.
We investigated the effect HDM extract on the release of pro-angiogenic and proinflammatory cytokines from BSMC.
Methods.
Human primary BSMC were stimulated with HDM extract in the absence or presence of fetal calf serum (FCS).
Twenty angiogenic cytokines were detected by a specific antibody array and modified protein levels were confirmed by ELISA.
Neutrophil migration was measured using a 96-well Boyden chamber.
Results.
ENA-78/CXCL5 protein levels in conditioned medium of BSMC stimulated with HDM extract were significantly reduced (n=10, P<0.05) but restored in the presence of 5% FCS.
HDM extracts did not affect ENA-78/CXCL5 mRNA levels.
Recombinant ENA-78/CXCL5 was degraded after incubation with HDM extracts (n=7, P<0.05) but restored after the addition of the serine protease AEBSF.
Neutrophil migration towards recombinant ENA-78/CXCL5 was also reduced in the presence of HDM extract.
Conclusion.
HDM proteases degrade ENA-78/CXCL5.
Thus exposure to HDM allergens may alter ENA-78/CXCL5 levels in the lungs and may affect angiogenesis and the inflammatory response in the airways of asthma patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Keglowich, Laura& Tamm, Michael& Zhong, Jun& Miglino, Nicola& Borger, Pieter. 2014. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-489420
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Keglowich, Laura…[et al.]. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-489420
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Keglowich, Laura& Tamm, Michael& Zhong, Jun& Miglino, Nicola& Borger, Pieter. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-489420
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489420
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر