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Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration
Joint Authors
Borger, Pieter
Zhong, Jun
Tamm, Michael
Miglino, Nicola
Keglowich, Laura
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Bronchial smooth muscle cells (BSMC) are a major source of proinflammatory and proangiogenic cytokines and chemokines, including VEGF and CXC-chemokines.
CXC-chemokines act primarily on neutrophils, mediating their recruitment to and activation at the site of inflammation.
In humans, house-dust mite (HDM) allergens can cause asthmatic exacerbations and trigger an inflammatory response through protease-dependent mechanisms.
Objective.
We investigated the effect HDM extract on the release of pro-angiogenic and proinflammatory cytokines from BSMC.
Methods.
Human primary BSMC were stimulated with HDM extract in the absence or presence of fetal calf serum (FCS).
Twenty angiogenic cytokines were detected by a specific antibody array and modified protein levels were confirmed by ELISA.
Neutrophil migration was measured using a 96-well Boyden chamber.
Results.
ENA-78/CXCL5 protein levels in conditioned medium of BSMC stimulated with HDM extract were significantly reduced (n=10, P<0.05) but restored in the presence of 5% FCS.
HDM extracts did not affect ENA-78/CXCL5 mRNA levels.
Recombinant ENA-78/CXCL5 was degraded after incubation with HDM extracts (n=7, P<0.05) but restored after the addition of the serine protease AEBSF.
Neutrophil migration towards recombinant ENA-78/CXCL5 was also reduced in the presence of HDM extract.
Conclusion.
HDM proteases degrade ENA-78/CXCL5.
Thus exposure to HDM allergens may alter ENA-78/CXCL5 levels in the lungs and may affect angiogenesis and the inflammatory response in the airways of asthma patients.
American Psychological Association (APA)
Keglowich, Laura& Tamm, Michael& Zhong, Jun& Miglino, Nicola& Borger, Pieter. 2014. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-489420
Modern Language Association (MLA)
Keglowich, Laura…[et al.]. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-489420
American Medical Association (AMA)
Keglowich, Laura& Tamm, Michael& Zhong, Jun& Miglino, Nicola& Borger, Pieter. Proteolytic Activity Present in House-Dust-Mite Extracts Degrades ENA-78CXCL5 and Reduces Neutrophil Migration. Journal of Allergy. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-489420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489420