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Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies
المؤلفون المشاركون
Hung, Wei-Ting
Lin, Chun-Mao
Yang, Wen-Bin
Kao, Pai-Feng
Wang, Shwu-Huey
Liao, Yu-Han
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-12-06
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The major cell wall constituent of Ganoderma lucidum (G.
lucidum) is β-1,3-glucan.
This study examined the polysaccharide from the residues of alkaline-extracted fruiting bodies using high-performance anion-exchange chromatography (HPAEC), and it employed nuclear magnetic resonance (NMR) and mass spectrometry (MS) to confirm the structures.
We have successfully isolated low-molecular-weight β-1,3-glucan (LMG), in high yields, from the waste residue of extracted fruiting bodies of G.
lucidum.
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay evaluated the capability of LMG to suppress H2O2-induced cell death in RAW264.7 cells, identifying that LMG protected cells from H2O2-induced damage.
LMG treatment decreased H2O2-induced intracellular reactive oxygen species (ROS) production.
LMG also influenced sphingomyelinase (SMase) activity, stimulated by cell death to induce ceramide formation, and then increase cell ROS production.
Estimation of the activities of neutral and acid SMases in vitro showed that LMG suppressed the activities of both neutral and acid SMases in a concentration-dependent manner.
These results suggest that LMG, a water-soluble β-1,3-glucan recycled from extracted residue of G.
lucidum, possesses antioxidant capability against H2O2-induced cell death by attenuating intracellular ROS and inhibiting SMase activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kao, Pai-Feng& Wang, Shwu-Huey& Hung, Wei-Ting& Liao, Yu-Han& Lin, Chun-Mao& Yang, Wen-Bin. 2011. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489429
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kao, Pai-Feng…[et al.]. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-489429
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kao, Pai-Feng& Wang, Shwu-Huey& Hung, Wei-Ting& Liao, Yu-Han& Lin, Chun-Mao& Yang, Wen-Bin. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489429
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-489429
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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