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Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies
Joint Authors
Hung, Wei-Ting
Lin, Chun-Mao
Yang, Wen-Bin
Kao, Pai-Feng
Wang, Shwu-Huey
Liao, Yu-Han
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The major cell wall constituent of Ganoderma lucidum (G.
lucidum) is β-1,3-glucan.
This study examined the polysaccharide from the residues of alkaline-extracted fruiting bodies using high-performance anion-exchange chromatography (HPAEC), and it employed nuclear magnetic resonance (NMR) and mass spectrometry (MS) to confirm the structures.
We have successfully isolated low-molecular-weight β-1,3-glucan (LMG), in high yields, from the waste residue of extracted fruiting bodies of G.
lucidum.
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay evaluated the capability of LMG to suppress H2O2-induced cell death in RAW264.7 cells, identifying that LMG protected cells from H2O2-induced damage.
LMG treatment decreased H2O2-induced intracellular reactive oxygen species (ROS) production.
LMG also influenced sphingomyelinase (SMase) activity, stimulated by cell death to induce ceramide formation, and then increase cell ROS production.
Estimation of the activities of neutral and acid SMases in vitro showed that LMG suppressed the activities of both neutral and acid SMases in a concentration-dependent manner.
These results suggest that LMG, a water-soluble β-1,3-glucan recycled from extracted residue of G.
lucidum, possesses antioxidant capability against H2O2-induced cell death by attenuating intracellular ROS and inhibiting SMase activity.
American Psychological Association (APA)
Kao, Pai-Feng& Wang, Shwu-Huey& Hung, Wei-Ting& Liao, Yu-Han& Lin, Chun-Mao& Yang, Wen-Bin. 2011. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489429
Modern Language Association (MLA)
Kao, Pai-Feng…[et al.]. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-489429
American Medical Association (AMA)
Kao, Pai-Feng& Wang, Shwu-Huey& Hung, Wei-Ting& Liao, Yu-Han& Lin, Chun-Mao& Yang, Wen-Bin. Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489429
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489429