Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains

المؤلفون المشاركون

Krzymińska, Sylwia
Kaznowski, Adam
Ochocka, Katarzyna

المصدر

The Scientific World Journal

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-05-02

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب

الملخص EN

Serratia marcescens strains are opportunistic pathogens that are increasingly recognized as a cause of severe nosocomial infections.

In this study we observed interactions between nonpigmented strains with human epithelial and macrophage-like cells.

The strains revealed hemolytic activity only after the contact of the cells with erythrocytes.

The contact of the bacteria with the host cells was also essential to their cytotoxicity.

Moreover, all strains revealed adherence ability and were invasive to epithelial cells.

Analyses of cellular morphology and DNA fragmentation of the HEp-2 and J774 cells exhibited typical features of cells undergoing apoptosis.

We observed morphological changes, including condensation of nuclear chromatin and formation of membrane-bound apoptotic bodies.

The lowest apoptotic index in HEp-2 cells did not exceed 25%, whereas the highest reached 59% at 24 h and 72% at 48 h after infection.

Most of the strains (60%) induced fragmentation of nuclear DNA.

The process depended on the activation of caspases, and was completely blocked by the pan-caspase inhibitor z-VAD-fmk.

This study provided new insights into the mechanisms of nonpigmented S.

marcescens pathogenesis.

The results revealed that the strains produce cell-contact toxins that facilitate bacterial invasion, induce hemolysis, cytotoxicity, and apoptosis of host cells.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Krzymińska, Sylwia& Ochocka, Katarzyna& Kaznowski, Adam. 2012. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489902

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Krzymińska, Sylwia…[et al.]. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-489902

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Krzymińska, Sylwia& Ochocka, Katarzyna& Kaznowski, Adam. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489902

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-489902