Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains
Joint Authors
Krzymińska, Sylwia
Kaznowski, Adam
Ochocka, Katarzyna
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Serratia marcescens strains are opportunistic pathogens that are increasingly recognized as a cause of severe nosocomial infections.
In this study we observed interactions between nonpigmented strains with human epithelial and macrophage-like cells.
The strains revealed hemolytic activity only after the contact of the cells with erythrocytes.
The contact of the bacteria with the host cells was also essential to their cytotoxicity.
Moreover, all strains revealed adherence ability and were invasive to epithelial cells.
Analyses of cellular morphology and DNA fragmentation of the HEp-2 and J774 cells exhibited typical features of cells undergoing apoptosis.
We observed morphological changes, including condensation of nuclear chromatin and formation of membrane-bound apoptotic bodies.
The lowest apoptotic index in HEp-2 cells did not exceed 25%, whereas the highest reached 59% at 24 h and 72% at 48 h after infection.
Most of the strains (60%) induced fragmentation of nuclear DNA.
The process depended on the activation of caspases, and was completely blocked by the pan-caspase inhibitor z-VAD-fmk.
This study provided new insights into the mechanisms of nonpigmented S.
marcescens pathogenesis.
The results revealed that the strains produce cell-contact toxins that facilitate bacterial invasion, induce hemolysis, cytotoxicity, and apoptosis of host cells.
American Psychological Association (APA)
Krzymińska, Sylwia& Ochocka, Katarzyna& Kaznowski, Adam. 2012. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489902
Modern Language Association (MLA)
Krzymińska, Sylwia…[et al.]. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-489902
American Medical Association (AMA)
Krzymińska, Sylwia& Ochocka, Katarzyna& Kaznowski, Adam. Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-489902
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-489902