Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis

الملخص EN

This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas.

By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometrial carcinomas and 12 normal endometrial samples was examined.

Methylated allele was found in 37.5% (27/72) of endometrial carcinomas but only in 8.3% (1/12) of normal endometrial, significantly more frequent in cancers (P=.0472).

SOX2 mRNA level was significantly reduced in endometrial carcinoma compared with nonneoplastic endometrium (P=.045).

A significant correlation between SOX2 mRNA expression and hypermethylation of SOX2 was found (P=.024).

Hypermethylation of SOX2 tended to be more frequently found in type II serous or clear cell adenocarcinoma.

SOX2 methylation was also significantly correlated with shorter survival of patients (P=.046).

In conclusion, epigenetic mechanisms may play a crucial role on the transcriptional regulation of SOX2 and loss of SOX2 expression may be related to endometrial carcinogenesis.