Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles
المؤلفون المشاركون
Lin, Po-Chiao
Hsieh, Chiung-wen
Hsieh, Shuchen
Wu, Chun-Hsin
Hsieh, Shu-Ling
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-01
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Efficient maintenance of glucose homeostasis is a major challenge in diabetes therapy, where accurate and reliable glucose level detection is required.
Though several methods are currently used, these suffer from impaired response and often unpredictable drift, making them unsuitable for long-term therapeutic practice.
In this study, we demonstrate a method that uses a functionalized atomic force microscope (AFM) cantilever as the sensor for reliable glucose detection with sufficient sensitivity and selectivity for clinical use.
We first modified the AFM tip with aminopropylsilatrane (APS) and then adsorbed glucose-specific lectin concanavalin A (Con A) onto the surface.
The Con A/APS-modified probes were then used to detect glucose by monitoring shifts in the cantilever resonance frequency.
To confirm the molecule-specific interaction, AFM topographical images were acquired of identically treated silicon substrates which indicated a specific attachment for glucose-Con A and not for galactose-Con A.
These results demonstrate that by monitoring the frequency shift of the AFM cantilever, this sensing system can detect the interaction between Con A and glucose, one of the biomolecule recognition processes, and may assist in the detection and mass quantification of glucose for clinical applications with very high sensitivity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hsieh, Shuchen& Hsieh, Shu-Ling& Hsieh, Chiung-wen& Lin, Po-Chiao& Wu, Chun-Hsin. 2013. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-490626
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hsieh, Shuchen…[et al.]. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-490626
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hsieh, Shuchen& Hsieh, Shu-Ling& Hsieh, Chiung-wen& Lin, Po-Chiao& Wu, Chun-Hsin. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-490626
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-490626
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر