![](/images/graphics-bg.png)
Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles
Joint Authors
Lin, Po-Chiao
Hsieh, Chiung-wen
Hsieh, Shuchen
Wu, Chun-Hsin
Hsieh, Shu-Ling
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-01
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Efficient maintenance of glucose homeostasis is a major challenge in diabetes therapy, where accurate and reliable glucose level detection is required.
Though several methods are currently used, these suffer from impaired response and often unpredictable drift, making them unsuitable for long-term therapeutic practice.
In this study, we demonstrate a method that uses a functionalized atomic force microscope (AFM) cantilever as the sensor for reliable glucose detection with sufficient sensitivity and selectivity for clinical use.
We first modified the AFM tip with aminopropylsilatrane (APS) and then adsorbed glucose-specific lectin concanavalin A (Con A) onto the surface.
The Con A/APS-modified probes were then used to detect glucose by monitoring shifts in the cantilever resonance frequency.
To confirm the molecule-specific interaction, AFM topographical images were acquired of identically treated silicon substrates which indicated a specific attachment for glucose-Con A and not for galactose-Con A.
These results demonstrate that by monitoring the frequency shift of the AFM cantilever, this sensing system can detect the interaction between Con A and glucose, one of the biomolecule recognition processes, and may assist in the detection and mass quantification of glucose for clinical applications with very high sensitivity.
American Psychological Association (APA)
Hsieh, Shuchen& Hsieh, Shu-Ling& Hsieh, Chiung-wen& Lin, Po-Chiao& Wu, Chun-Hsin. 2013. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-490626
Modern Language Association (MLA)
Hsieh, Shuchen…[et al.]. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-490626
American Medical Association (AMA)
Hsieh, Shuchen& Hsieh, Shu-Ling& Hsieh, Chiung-wen& Lin, Po-Chiao& Wu, Chun-Hsin. Label-Free Glucose Detection Using Cantilever Sensor Technology Based on Gravimetric Detection Principles. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-490626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-490626