Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells

الملخص EN

Objective.

To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1).

Methods.

TEV-1 cells were incubated with different doses of peptidoglycan.

The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining.

Results.

TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane.

Compared with the untreated control, TEV-1 cells incubated with 30 μg/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (P<.01).

However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P>.05) after 24 hours of treatment.

On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P>.05).

Conclusion.

Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells.

It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.