Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells
المؤلفون المشاركون
Wong, Wu Shun Felix
Lim, Chi Eung Danforn
Wang, Yi
Luo, Xi-Ping
Zhong, Gang
المصدر
ISRN Obstetrics and Gynecology
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-10-07
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1).
Methods.
TEV-1 cells were incubated with different doses of peptidoglycan.
The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining.
Results.
TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane.
Compared with the untreated control, TEV-1 cells incubated with 30 μg/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (P<.01).
However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P>.05) after 24 hours of treatment.
On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P>.05).
Conclusion.
Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells.
It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yi& Luo, Xi-Ping& Lim, Chi Eung Danforn& Wong, Wu Shun Felix& Zhong, Gang. 2010. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-491050
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yi…[et al.]. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-491050
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yi& Luo, Xi-Ping& Lim, Chi Eung Danforn& Wong, Wu Shun Felix& Zhong, Gang. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology. 2010. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-491050
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491050
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر