Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells
Joint Authors
Wong, Wu Shun Felix
Lim, Chi Eung Danforn
Wang, Yi
Luo, Xi-Ping
Zhong, Gang
Source
ISRN Obstetrics and Gynecology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-07
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
To investigate the regulatory effect of peptidoglycan on the expression of human Toll-like receptors 2 (TLR2) mRNA and proteins in the human extravillous trophoblast cell line (TEV-1).
Methods.
TEV-1 cells were incubated with different doses of peptidoglycan.
The expression of TLR2 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry SP staining.
Results.
TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane.
Compared with the untreated control, TEV-1 cells incubated with 30 μg/ml peptidoglycan significantly upregulated the expression of TLR2 mRNA and protein after 12 hours of treatment (P<.01).
However, the expression of TLR2 mRNA and protein was decreased but had no significant difference compared with the control (P>.05) after 24 hours of treatment.
On the other hand, 10 μg/ml peptidoglycan did not seem to have regulatory effect on mRNA and protein expression of TLR2 (P>.05).
Conclusion.
Peptidoglycan has a role in regulating the expression of TLR2 mRNA and protein in TEV-1 cells.
It suggests that the trophoblast cells may play important role in the immune response at the fetal-maternal interface and affect the result of pregnancy by expressing TLR2.
American Psychological Association (APA)
Wang, Yi& Luo, Xi-Ping& Lim, Chi Eung Danforn& Wong, Wu Shun Felix& Zhong, Gang. 2010. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-491050
Modern Language Association (MLA)
Wang, Yi…[et al.]. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-491050
American Medical Association (AMA)
Wang, Yi& Luo, Xi-Ping& Lim, Chi Eung Danforn& Wong, Wu Shun Felix& Zhong, Gang. Regulatory Effect of Peptidoglycan on the Expression of Toll-Like Receptor 2 mRNA and Proteins in Trophoblast Cell Line TEV-1 Cells. ISRN Obstetrics and Gynecology. 2010. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-491050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491050