ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury
المؤلفون المشاركون
Akyol, Sumeyya
Yigitoglu, M. Ramazan
McCulloch, Daniel R.
Yonezawa, Tomoko
Ugurcu, Veli
Akyol, Omer
Demircan, Kadir
Hirohata, Satoshi
Ozturk, Gulfer
Takigawa, Tomoyuki
Topcu, Vehap
Hasgul, Rukiye
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The chondroitin sulfate proteoglycans (CSPGs) aggrecan, versican, and brevican are large aggregating extracellular matrix molecules that inhibit axonal growth of the mature central nervous system (CNS).
ADAMTS proteoglycanases, including ADAMTS4 and ADAMTS5, degrade CSPGs, representing potential targets for ameliorating axonal growth-inhibition by CSPG accumulation after CNS injury.
We investigated the proteolysis of CSPGs in mice homozygous for Adamts4 or Adamts5 null alleles after spinal cord injury (SCI).
ADAMTS-derived 50–60 kDa aggrecan and 50 kDa brevican fragments were observed in Adamts4−/−, Adamts5−/−, and wt mice but not in the sham-operated group.
By contrast Adamts4−/− and Adamts5−/− mice were both protected from versican proteolysis with an ADAMTS-generated 70 kDa versican fragment predominately observed in WT mice.
ADAMTS1, ADAMTS9, and ADAMTS15 were detected by Western blot in Adamts4−/− mice’ spinal cords after SCI.
Immunohistochemistry showed astrocyte accumulation at the injury site.
These data indicate that aggrecan and brevican proteolysis is compensated in Adamts4−/− or Adamts5−/− mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during SCI.
We show robust ADAMTS activity after SCI and exemplify the requirement for collective proteolysis for effective CSPG clearance during SCI.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Demircan, Kadir& Topcu, Vehap& Takigawa, Tomoyuki& Akyol, Sumeyya& Yonezawa, Tomoko& Ozturk, Gulfer…[et al.]. 2014. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491121
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Demircan, Kadir…[et al.]. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-491121
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Demircan, Kadir& Topcu, Vehap& Takigawa, Tomoyuki& Akyol, Sumeyya& Yonezawa, Tomoko& Ozturk, Gulfer…[et al.]. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491121
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491121
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر