ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury
Joint Authors
Akyol, Sumeyya
Yigitoglu, M. Ramazan
McCulloch, Daniel R.
Yonezawa, Tomoko
Ugurcu, Veli
Akyol, Omer
Demircan, Kadir
Hirohata, Satoshi
Ozturk, Gulfer
Takigawa, Tomoyuki
Topcu, Vehap
Hasgul, Rukiye
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The chondroitin sulfate proteoglycans (CSPGs) aggrecan, versican, and brevican are large aggregating extracellular matrix molecules that inhibit axonal growth of the mature central nervous system (CNS).
ADAMTS proteoglycanases, including ADAMTS4 and ADAMTS5, degrade CSPGs, representing potential targets for ameliorating axonal growth-inhibition by CSPG accumulation after CNS injury.
We investigated the proteolysis of CSPGs in mice homozygous for Adamts4 or Adamts5 null alleles after spinal cord injury (SCI).
ADAMTS-derived 50–60 kDa aggrecan and 50 kDa brevican fragments were observed in Adamts4−/−, Adamts5−/−, and wt mice but not in the sham-operated group.
By contrast Adamts4−/− and Adamts5−/− mice were both protected from versican proteolysis with an ADAMTS-generated 70 kDa versican fragment predominately observed in WT mice.
ADAMTS1, ADAMTS9, and ADAMTS15 were detected by Western blot in Adamts4−/− mice’ spinal cords after SCI.
Immunohistochemistry showed astrocyte accumulation at the injury site.
These data indicate that aggrecan and brevican proteolysis is compensated in Adamts4−/− or Adamts5−/− mice by ADAMTS proteoglycanase family members but a threshold of versican proteolysis is sensitive to the loss of a single ADAMTS proteoglycanase during SCI.
We show robust ADAMTS activity after SCI and exemplify the requirement for collective proteolysis for effective CSPG clearance during SCI.
American Psychological Association (APA)
Demircan, Kadir& Topcu, Vehap& Takigawa, Tomoyuki& Akyol, Sumeyya& Yonezawa, Tomoko& Ozturk, Gulfer…[et al.]. 2014. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491121
Modern Language Association (MLA)
Demircan, Kadir…[et al.]. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-491121
American Medical Association (AMA)
Demircan, Kadir& Topcu, Vehap& Takigawa, Tomoyuki& Akyol, Sumeyya& Yonezawa, Tomoko& Ozturk, Gulfer…[et al.]. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-491121
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491121