Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
المؤلفون المشاركون
Liang, Jy-Been
Chen, Jiann-Torng
Liang, Chang-Min
Lu, Da-Wen
Tai, Ming-Cheng
Chen, Ching-Long
Chang, Yun-Hsiang
Chen, Yi-Hao
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
To evaluate the effects of silibinin on intercellular adhesion molecule-1 (ICAM-1) expression, we used ARPE-19 cells as a model in which tumor necrosis factor (TNF-α) and interferon (IFN-γ) enhanced ICAM-1 expression.
This upregulation was inhibited by silibinin.
In an adherence assay using ARPE-19 and THP-1 cells, silibinin inhibited the cell adhesion function of ICAM-1.
The inhibitory effects of silibinin on ICAM-1 expression were mediated via the blockage of nuclear translocation of p65 proteins in TNF-α and phosphorylation of STAT1 in IFN-γ-stimulated cells.
In addition, silibinin altered the degree of N-linked glycosylation posttranslationally in ARPE-19 cells by significantly enhancing MGAT3 gene expression.
Silibinin can increase the O-GlcNAc levels of glycoproteins in ARPE-19 cells.
In a reporter gene assay, PUGNAc, which can also increase O-GlcNAc levels, inhibited NF-κB reporter activity in TNF-α-induced ARPE-19 cells and this process was augmented by silibinin treatment.
Overexpression of OGT gene was associated with reduced TNF-α-induced ICAM-1 levels, which is consistent with that induced by silibinin treatment.
Taken together, silibinin inhibits ICAM-1 expression and its function through altered O-linked glycosylation in NF-κB and STAT1 signaling pathways and decreases the N-linked glycosylation of ICAM-1 transmembrane protein in proinflammatory cytokine-stimulated ARPE-19 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Yi-Hao& Chen, Ching-Long& Liang, Chang-Min& Liang, Jy-Been& Tai, Ming-Cheng& Chang, Yun-Hsiang…[et al.]. 2014. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491619
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Yi-Hao…[et al.]. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-491619
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Yi-Hao& Chen, Ching-Long& Liang, Chang-Min& Liang, Jy-Been& Tai, Ming-Cheng& Chang, Yun-Hsiang…[et al.]. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491619
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-491619
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر