Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells
Joint Authors
Liang, Jy-Been
Chen, Jiann-Torng
Liang, Chang-Min
Lu, Da-Wen
Tai, Ming-Cheng
Chen, Ching-Long
Chang, Yun-Hsiang
Chen, Yi-Hao
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
To evaluate the effects of silibinin on intercellular adhesion molecule-1 (ICAM-1) expression, we used ARPE-19 cells as a model in which tumor necrosis factor (TNF-α) and interferon (IFN-γ) enhanced ICAM-1 expression.
This upregulation was inhibited by silibinin.
In an adherence assay using ARPE-19 and THP-1 cells, silibinin inhibited the cell adhesion function of ICAM-1.
The inhibitory effects of silibinin on ICAM-1 expression were mediated via the blockage of nuclear translocation of p65 proteins in TNF-α and phosphorylation of STAT1 in IFN-γ-stimulated cells.
In addition, silibinin altered the degree of N-linked glycosylation posttranslationally in ARPE-19 cells by significantly enhancing MGAT3 gene expression.
Silibinin can increase the O-GlcNAc levels of glycoproteins in ARPE-19 cells.
In a reporter gene assay, PUGNAc, which can also increase O-GlcNAc levels, inhibited NF-κB reporter activity in TNF-α-induced ARPE-19 cells and this process was augmented by silibinin treatment.
Overexpression of OGT gene was associated with reduced TNF-α-induced ICAM-1 levels, which is consistent with that induced by silibinin treatment.
Taken together, silibinin inhibits ICAM-1 expression and its function through altered O-linked glycosylation in NF-κB and STAT1 signaling pathways and decreases the N-linked glycosylation of ICAM-1 transmembrane protein in proinflammatory cytokine-stimulated ARPE-19 cells.
American Psychological Association (APA)
Chen, Yi-Hao& Chen, Ching-Long& Liang, Chang-Min& Liang, Jy-Been& Tai, Ming-Cheng& Chang, Yun-Hsiang…[et al.]. 2014. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491619
Modern Language Association (MLA)
Chen, Yi-Hao…[et al.]. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-491619
American Medical Association (AMA)
Chen, Yi-Hao& Chen, Ching-Long& Liang, Chang-Min& Liang, Jy-Been& Tai, Ming-Cheng& Chang, Yun-Hsiang…[et al.]. Silibinin Inhibits ICAM-1 Expression via Regulation of N-Linked and O-Linked Glycosylation in ARPE-19 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-491619
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-491619