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Morphological Measurement of Living Cells in Methanol with Digital Holographic Microscopy
المؤلفون المشاركون
Wang, Xinlong
Yang, Yishu
Zhang, Yizhuo
Wang, Yunxin
Wang, Dayong
Ouyang, Liting
Zhao, Jie
المصدر
Computational and Mathematical Methods in Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-01-27
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Cell morphology is the research foundation in many applications related to the estimation of cell status, drug response, and toxicity screening.
In biomedical field, the quantitative phase detection is an inevitable trend for living cells.
In this paper, the morphological change of HeLa cells treated with methanol of different concentrations is detected using digital holographic microscopy.
The compact image-plane digital holographic system is designed based on fiber elements.
The quantitative phase image of living cells is obtained in combination with numerical analysis.
The statistical analysis shows that the area and average optical thickness of HeLa cells treated with 12.5% or 25% methanol reduce significantly, which indicates that the methanol with lower concentration could cause cellular shrinkage.
The area of HeLa cells treated with 50% methanol is similar to that of normal cells (P>0.05), which reveals the fixative effect of methanol with higher concentration.
The maximum optical thickness of the cells treated with 12.5%, 25%, and 50% methanol is greater than that of untreated cells, which implies the pyknosis of HeLa cells under the effect of methanol.
All of the results demonstrate that digital holographic microscopy has supplied a noninvasive imaging alternative to measure the morphological change of label-free living cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Yunxin& Yang, Yishu& Wang, Dayong& Ouyang, Liting& Zhang, Yizhuo& Zhao, Jie…[et al.]. 2013. Morphological Measurement of Living Cells in Methanol with Digital Holographic Microscopy. Computational and Mathematical Methods in Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-492846
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Yunxin…[et al.]. Morphological Measurement of Living Cells in Methanol with Digital Holographic Microscopy. Computational and Mathematical Methods in Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-492846
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Yunxin& Yang, Yishu& Wang, Dayong& Ouyang, Liting& Zhang, Yizhuo& Zhao, Jie…[et al.]. Morphological Measurement of Living Cells in Methanol with Digital Holographic Microscopy. Computational and Mathematical Methods in Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-492846
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-492846
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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