Morphological Measurement of Living Cells in Methanol with Digital Holographic Microscopy

Abstract EN

Cell morphology is the research foundation in many applications related to the estimation of cell status, drug response, and toxicity screening.

In biomedical field, the quantitative phase detection is an inevitable trend for living cells.

In this paper, the morphological change of HeLa cells treated with methanol of different concentrations is detected using digital holographic microscopy.

The compact image-plane digital holographic system is designed based on fiber elements.

The quantitative phase image of living cells is obtained in combination with numerical analysis.

The statistical analysis shows that the area and average optical thickness of HeLa cells treated with 12.5% or 25% methanol reduce significantly, which indicates that the methanol with lower concentration could cause cellular shrinkage.

The area of HeLa cells treated with 50% methanol is similar to that of normal cells (P>0.05), which reveals the fixative effect of methanol with higher concentration.

The maximum optical thickness of the cells treated with 12.5%, 25%, and 50% methanol is greater than that of untreated cells, which implies the pyknosis of HeLa cells under the effect of methanol.

All of the results demonstrate that digital holographic microscopy has supplied a noninvasive imaging alternative to measure the morphological change of label-free living cells.