In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts
المؤلفون المشاركون
De Souza Costa, Carlos Alberto
Hebling, Josimeri
Bagnato, Vanderlei Salvador
Turrioni, Ana Paula S.
Pansani, Taisa N.
Basso, Fernanda G.
المصدر
International Journal of Dentistry
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-15
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture.
Cells (3×104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum.
After 48-hour incubation with 5% CO2 at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2.
Cells were irradiated every 24 h totalizing 3 applications.
Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay).
Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%.
Irradiation of the fibroblasts with 0.5 and 3 J/cm2 resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05).
Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05).
These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Basso, Fernanda G.& Pansani, Taisa N.& Turrioni, Ana Paula S.& Bagnato, Vanderlei Salvador& Hebling, Josimeri& De Souza Costa, Carlos Alberto. 2012. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-493169
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Basso, Fernanda G.…[et al.]. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-493169
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Basso, Fernanda G.& Pansani, Taisa N.& Turrioni, Ana Paula S.& Bagnato, Vanderlei Salvador& Hebling, Josimeri& De Souza Costa, Carlos Alberto. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-493169
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493169
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر