In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts
Joint Authors
De Souza Costa, Carlos Alberto
Hebling, Josimeri
Bagnato, Vanderlei Salvador
Turrioni, Ana Paula S.
Pansani, Taisa N.
Basso, Fernanda G.
Source
International Journal of Dentistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of this study was to determine adequate energy doses using specific parameters of LLLT to produce biostimulatory effects on human gingival fibroblast culture.
Cells (3×104 cells/cm2) were seeded on 24-well acrylic plates using plain DMEM supplemented with 10% fetal bovine serum.
After 48-hour incubation with 5% CO2 at 37°C, cells were irradiated with a InGaAsP diode laser prototype (LASERTable; 780±3 nm; 40 mW) with energy doses of 0.5, 1.5, 3, 5, and 7 J/cm2.
Cells were irradiated every 24 h totalizing 3 applications.
Twenty-four hours after the last irradiation, cell metabolism was evaluated by the MTT assay and the two most effective doses (0.5 and 3 J/cm2) were selected to evaluate the cell number (trypan blue assay) and the cell migration capacity (wound healing assay; transwell migration assay).
Data were analyzed by the Kruskal-Wallis and Mann-Whitney nonparametric tests with statistical significance of 5%.
Irradiation of the fibroblasts with 0.5 and 3 J/cm2 resulted in significant increase in cell metabolism compared with the nonrradiated group (P<0.05).
Both energy doses promoted significant increase in the cell number as well as in cell migration (P<0.05).
These results demonstrate that, under the tested conditions, LLLT promoted biostimulation of fibroblasts in vitro.
American Psychological Association (APA)
Basso, Fernanda G.& Pansani, Taisa N.& Turrioni, Ana Paula S.& Bagnato, Vanderlei Salvador& Hebling, Josimeri& De Souza Costa, Carlos Alberto. 2012. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-493169
Modern Language Association (MLA)
Basso, Fernanda G.…[et al.]. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-493169
American Medical Association (AMA)
Basso, Fernanda G.& Pansani, Taisa N.& Turrioni, Ana Paula S.& Bagnato, Vanderlei Salvador& Hebling, Josimeri& De Souza Costa, Carlos Alberto. In Vitro Wound Healing Improvement by Low-Level Laser Therapy Application in Cultured Gingival Fibroblasts. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-493169
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493169