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Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus
المؤلفون المشاركون
Yan, Shirong
Guan, Xueping
Pan, Yunjun
Qu, Xin
Zou, Li
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-16
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
Objectives.
To study the correlation between the methylation of protein kinase C epsilon zeta (PRKCZ) gene promoters and type 2 diabetes mellitus (T2DM).
Methods.
The case-control method was implemented in 272 unrelated to one another cases in Shiyan People’s Hospital.
Of those, 152 were diagnosed as T2DM cases, and the other 120 cases were healthy individuals visiting the hospital for a physical examination.
The subjects were first divided into two groups: the T2DM group and the normal control (NC) group.
Next, methylated DNA immunoprecipitation chip (MeDIP-chip) was used for detection.
Bisulfite sequencing PCR (BSP) and gene sequencing were then performed to detect and analyze the correlation between PRKCZ gene promoter methylation and T2DM.
Finally, Western blotting was applied to determine the serum level of PRKCZ.
The data were then analyzed with the statistics analyzing software SPSS 17.0.
Results.
In contrast with cases in NC, T2DM patients showed a high level of methylation, with 7 of 9 CpG sites were shown to be methylated, whereas, in the control group, only one CpG site was found to be methylated.
The methylated CpG sites for the two groups showed marked differences (P<0.01).
Additionally, the level of PRKCZ was decreased in T2DM subjects, and the difference between the two groups was statistically significant (P<0.05).
Discussion.
This study suggests that the PRKCZ gene is the hypermethylated gene of T2DM and the hypermethylation PRKCZ gene may be involved in the pathogenesis of T2DM.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zou, Li& Yan, Shirong& Guan, Xueping& Pan, Yunjun& Qu, Xin. 2013. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-493343
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zou, Li…[et al.]. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-493343
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zou, Li& Yan, Shirong& Guan, Xueping& Pan, Yunjun& Qu, Xin. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-493343
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493343
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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