Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus
Joint Authors
Yan, Shirong
Guan, Xueping
Pan, Yunjun
Qu, Xin
Zou, Li
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-16
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Objectives.
To study the correlation between the methylation of protein kinase C epsilon zeta (PRKCZ) gene promoters and type 2 diabetes mellitus (T2DM).
Methods.
The case-control method was implemented in 272 unrelated to one another cases in Shiyan People’s Hospital.
Of those, 152 were diagnosed as T2DM cases, and the other 120 cases were healthy individuals visiting the hospital for a physical examination.
The subjects were first divided into two groups: the T2DM group and the normal control (NC) group.
Next, methylated DNA immunoprecipitation chip (MeDIP-chip) was used for detection.
Bisulfite sequencing PCR (BSP) and gene sequencing were then performed to detect and analyze the correlation between PRKCZ gene promoter methylation and T2DM.
Finally, Western blotting was applied to determine the serum level of PRKCZ.
The data were then analyzed with the statistics analyzing software SPSS 17.0.
Results.
In contrast with cases in NC, T2DM patients showed a high level of methylation, with 7 of 9 CpG sites were shown to be methylated, whereas, in the control group, only one CpG site was found to be methylated.
The methylated CpG sites for the two groups showed marked differences (P<0.01).
Additionally, the level of PRKCZ was decreased in T2DM subjects, and the difference between the two groups was statistically significant (P<0.05).
Discussion.
This study suggests that the PRKCZ gene is the hypermethylated gene of T2DM and the hypermethylation PRKCZ gene may be involved in the pathogenesis of T2DM.
American Psychological Association (APA)
Zou, Li& Yan, Shirong& Guan, Xueping& Pan, Yunjun& Qu, Xin. 2013. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-493343
Modern Language Association (MLA)
Zou, Li…[et al.]. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-493343
American Medical Association (AMA)
Zou, Li& Yan, Shirong& Guan, Xueping& Pan, Yunjun& Qu, Xin. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-493343
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493343