Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
المؤلفون المشاركون
Vijayaraghavan, Ponnuswamy
Prakash Vincent, Samuel Gnana
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-09
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice.
Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme.
Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach.
Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influenced enzyme production (P<0.05).
A central composite design resulted in the production of 3699 U/mL of enzyme in the presence of 0.3% (w/w) beef extract and 0.05% (w/w) sodium dihydrogen phosphate, at 100% (v/w) moisture after 72 h of fermentation.
The enzyme production increased fourfold compared to the original medium.
This enzyme was purified to homogeneity by ammonium sulfate precipitation, diethylaminoethyl-cellulose ion-exchange chromatography, Sephadex G-75 gel filtration chromatography, and casein-agarose affinity chromatography and had an apparent molecular mass of 29.5 kDa.
The optimum pH and temperature for the activity of fibrinolytic enzyme were found to be 8.0 and 60°C, respectively.
This enzyme was highly stable at wide pH range (7.0–9.0) and showed 27% ± 6% enzyme activity after initial denaturation at 60°C for 1 h.
In vitro assays revealed that the enzyme could activate plasminogen and significantly degraded the fibrin net of blood clot, which suggests its potential as an effective thrombolytic agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. 2014. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-493600
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-493600
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-493600
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493600
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر