Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
Joint Authors
Vijayaraghavan, Ponnuswamy
Prakash Vincent, Samuel Gnana
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice.
Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme.
Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach.
Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influenced enzyme production (P<0.05).
A central composite design resulted in the production of 3699 U/mL of enzyme in the presence of 0.3% (w/w) beef extract and 0.05% (w/w) sodium dihydrogen phosphate, at 100% (v/w) moisture after 72 h of fermentation.
The enzyme production increased fourfold compared to the original medium.
This enzyme was purified to homogeneity by ammonium sulfate precipitation, diethylaminoethyl-cellulose ion-exchange chromatography, Sephadex G-75 gel filtration chromatography, and casein-agarose affinity chromatography and had an apparent molecular mass of 29.5 kDa.
The optimum pH and temperature for the activity of fibrinolytic enzyme were found to be 8.0 and 60°C, respectively.
This enzyme was highly stable at wide pH range (7.0–9.0) and showed 27% ± 6% enzyme activity after initial denaturation at 60°C for 1 h.
In vitro assays revealed that the enzyme could activate plasminogen and significantly degraded the fibrin net of blood clot, which suggests its potential as an effective thrombolytic agent.
American Psychological Association (APA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. 2014. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-493600
Modern Language Association (MLA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-493600
American Medical Association (AMA)
Vijayaraghavan, Ponnuswamy& Prakash Vincent, Samuel Gnana. Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-493600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493600