Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium
المؤلفون المشاركون
Granzier, Henk L. M.
Fukushima, Hideto
Chung, Charles S.
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-04-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Titin, also known as connectin, is a large filamentous protein that greatly contributes to passive myocardial stiffness.
In vitro evidence suggests that one of titin's spring elements, the PEVK, interacts with actin and that this adds a viscous component to passive stiffness.
Differential splicing of titin gives rise to the stiff N2B and more compliant N2BA isoforms.
Here we studied the titin-isoform dependence of titin-actin interaction and studied the bovine left atrium (BLA) that expresses mainly N2BA titin, and the bovine left ventricle (BLV) that expresses a mixture of both N2B and N2BA isforms.
For comparison we also studied mouse left ventricular (MLV) myocardium which expresses predominately N2B titin.
Using the actin-severing protein gelsolin, we obtained evidence that titin-actin interaction contributes significantly to passive myocardial stiffness in all tissue types, but most in MLV, least in BLA, and an intermediate level in BLV.
We also studied whether titin-actin interaction is regulated by S100A1/calcium and found that calcium alone or S100A1 alone did not alter passive stiffness, but that combined they significantly lowered stiffness.
We propose that titin-actin interaction is a “viscous break” that is on during diastole and off during systole.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fukushima, Hideto& Chung, Charles S.& Granzier, Henk L. M.. 2010. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-493830
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fukushima, Hideto…[et al.]. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-493830
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fukushima, Hideto& Chung, Charles S.& Granzier, Henk L. M.. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-493830
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493830
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر