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Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium
Joint Authors
Granzier, Henk L. M.
Fukushima, Hideto
Chung, Charles S.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Titin, also known as connectin, is a large filamentous protein that greatly contributes to passive myocardial stiffness.
In vitro evidence suggests that one of titin's spring elements, the PEVK, interacts with actin and that this adds a viscous component to passive stiffness.
Differential splicing of titin gives rise to the stiff N2B and more compliant N2BA isoforms.
Here we studied the titin-isoform dependence of titin-actin interaction and studied the bovine left atrium (BLA) that expresses mainly N2BA titin, and the bovine left ventricle (BLV) that expresses a mixture of both N2B and N2BA isforms.
For comparison we also studied mouse left ventricular (MLV) myocardium which expresses predominately N2B titin.
Using the actin-severing protein gelsolin, we obtained evidence that titin-actin interaction contributes significantly to passive myocardial stiffness in all tissue types, but most in MLV, least in BLA, and an intermediate level in BLV.
We also studied whether titin-actin interaction is regulated by S100A1/calcium and found that calcium alone or S100A1 alone did not alter passive stiffness, but that combined they significantly lowered stiffness.
We propose that titin-actin interaction is a “viscous break” that is on during diastole and off during systole.
American Psychological Association (APA)
Fukushima, Hideto& Chung, Charles S.& Granzier, Henk L. M.. 2010. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-493830
Modern Language Association (MLA)
Fukushima, Hideto…[et al.]. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-493830
American Medical Association (AMA)
Fukushima, Hideto& Chung, Charles S.& Granzier, Henk L. M.. Titin-Isoform Dependence of Titin-Actin Interaction and Its Regulation by S100A1Ca2+ in Skinned Myocardium. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-493830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-493830