Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala

المؤلفون المشاركون

Rahman, Khalid
Li, Qing-Shan
Guo, Dai-Hong
Li, Jie
Chen, Xu
Hu, Yuan
Liu, Ping

المصدر

Journal of Biomedicine and Biotechnology

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-08-03

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الطب البشري

الملخص EN

The neuroprotective effects of 3,6′-disinapoyl sucrose (DISS) from Radix Polygala against glutamate-induced SH-SY5Y neuronal cells injury were evaluated in the present study.

SH-SY5Y neuronal cells were pretreated with glutamate (8 mM) for 30 min followed by cotreatment with DISS for 12 h.

Cell viability was determined by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay, and apoptosis was confirmed by cell morphology and flow cytometry assay, evaluated with propidium iodide dye.

Treatment with DISS (0.6, 6, and 60 μmol/L) increased cell viability dose dependently, inhibited LDH release, and attenuated apoptosis.

The mechanisms by which DISS protected neuron cells from glutamate-induced excitotoxicity included the downregulation of proapoptotic gene Bax and the upregulation of antiapoptotic gene Bcl-2.

The present findings indicated that DISS exerts neuroprotective effects against glutamate toxicity, which might be of importance and contribute to its clinical efficacy for the treatment of neurodegenerative diseases.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hu, Yuan& Li, Jie& Liu, Ping& Chen, Xu& Guo, Dai-Hong& Li, Qing-Shan…[et al.]. 2011. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-493911

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hu, Yuan…[et al.]. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-493911

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hu, Yuan& Li, Jie& Liu, Ping& Chen, Xu& Guo, Dai-Hong& Li, Qing-Shan…[et al.]. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-493911

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-493911