Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala

Joint Authors

Rahman, Khalid
Li, Qing-Shan
Guo, Dai-Hong
Li, Jie
Chen, Xu
Hu, Yuan
Liu, Ping

Source

Journal of Biomedicine and Biotechnology

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-08-03

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Medicine

Abstract EN

The neuroprotective effects of 3,6′-disinapoyl sucrose (DISS) from Radix Polygala against glutamate-induced SH-SY5Y neuronal cells injury were evaluated in the present study.

SH-SY5Y neuronal cells were pretreated with glutamate (8 mM) for 30 min followed by cotreatment with DISS for 12 h.

Cell viability was determined by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay, and apoptosis was confirmed by cell morphology and flow cytometry assay, evaluated with propidium iodide dye.

Treatment with DISS (0.6, 6, and 60 μmol/L) increased cell viability dose dependently, inhibited LDH release, and attenuated apoptosis.

The mechanisms by which DISS protected neuron cells from glutamate-induced excitotoxicity included the downregulation of proapoptotic gene Bax and the upregulation of antiapoptotic gene Bcl-2.

The present findings indicated that DISS exerts neuroprotective effects against glutamate toxicity, which might be of importance and contribute to its clinical efficacy for the treatment of neurodegenerative diseases.

American Psychological Association (APA)

Hu, Yuan& Li, Jie& Liu, Ping& Chen, Xu& Guo, Dai-Hong& Li, Qing-Shan…[et al.]. 2011. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-493911

Modern Language Association (MLA)

Hu, Yuan…[et al.]. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-493911

American Medical Association (AMA)

Hu, Yuan& Li, Jie& Liu, Ping& Chen, Xu& Guo, Dai-Hong& Li, Qing-Shan…[et al.]. Protection of SH-SY5Y Neuronal Cells from Glutamate-Induced Apoptosis by 3,6′-Disinapoyl Sucrose, a Bioactive Compound Isolated from Radix Polygala. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-493911

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-493911