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Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling
المؤلفون المشاركون
Yuqin, Li
Jia, Baoxiu
Yurong, Guo
Caihong, Liu
Zhen, Yang
Jiao, Chen
You, Guirong
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The interaction of patulin with human serum albumin (HSA) was studied in vitro under normal physiological conditions.
The study was performed using fluorescence, ultraviolet-visible spectroscopy (UV-Vis), circular dichroism (CD), atomic force microscopy (AFM), and molecular modeling techniques.
The quenching mechanism was investigated using the association constants, the number of binding sites, and basic thermodynamic parameters.
A dynamic quenching mechanism occurred between HSA and patulin, and the binding constants (K) were 2.60 × 104, 4.59 × 104, and 7.01 × 104 M−1 at 288, 300, and 310 K, respectively.
Based on fluorescence resonance energy transfer, the distance between the HSA and patulin was determined to be 2.847 nm.
The ΔG0, ΔH0, and ΔS0 values across various temperatures indicated that hydrophobic interaction was the predominant binding force.
The UV-Vis and CD results confirmed that the secondary structure of HSA was altered in the presence of patulin.
The AFM results revealed that the individual HSA molecule dimensions were larger after interaction with patulin.
In addition, molecular modeling showed that the patulin-HSA complex was stabilized by hydrophobic and hydrogen bond forces.
The study results suggested that a weak intermolecular interaction occurred between patulin and HSA.
Overall, the results are potentially useful for elucidating the toxigenicity of patulin when it is combined with the biomolecular function effect, transmembrane transport, toxicological, testing and other experiments.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yuqin, Li& You, Guirong& Zhen, Yang& Caihong, Liu& Jia, Baoxiu& Jiao, Chen…[et al.]. 2014. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494438
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yuqin, Li…[et al.]. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-494438
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yuqin, Li& You, Guirong& Zhen, Yang& Caihong, Liu& Jia, Baoxiu& Jiao, Chen…[et al.]. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494438
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-494438
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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