Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling
Joint Authors
Yuqin, Li
Jia, Baoxiu
Yurong, Guo
Caihong, Liu
Zhen, Yang
Jiao, Chen
You, Guirong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The interaction of patulin with human serum albumin (HSA) was studied in vitro under normal physiological conditions.
The study was performed using fluorescence, ultraviolet-visible spectroscopy (UV-Vis), circular dichroism (CD), atomic force microscopy (AFM), and molecular modeling techniques.
The quenching mechanism was investigated using the association constants, the number of binding sites, and basic thermodynamic parameters.
A dynamic quenching mechanism occurred between HSA and patulin, and the binding constants (K) were 2.60 × 104, 4.59 × 104, and 7.01 × 104 M−1 at 288, 300, and 310 K, respectively.
Based on fluorescence resonance energy transfer, the distance between the HSA and patulin was determined to be 2.847 nm.
The ΔG0, ΔH0, and ΔS0 values across various temperatures indicated that hydrophobic interaction was the predominant binding force.
The UV-Vis and CD results confirmed that the secondary structure of HSA was altered in the presence of patulin.
The AFM results revealed that the individual HSA molecule dimensions were larger after interaction with patulin.
In addition, molecular modeling showed that the patulin-HSA complex was stabilized by hydrophobic and hydrogen bond forces.
The study results suggested that a weak intermolecular interaction occurred between patulin and HSA.
Overall, the results are potentially useful for elucidating the toxigenicity of patulin when it is combined with the biomolecular function effect, transmembrane transport, toxicological, testing and other experiments.
American Psychological Association (APA)
Yuqin, Li& You, Guirong& Zhen, Yang& Caihong, Liu& Jia, Baoxiu& Jiao, Chen…[et al.]. 2014. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494438
Modern Language Association (MLA)
Yuqin, Li…[et al.]. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-494438
American Medical Association (AMA)
Yuqin, Li& You, Guirong& Zhen, Yang& Caihong, Liu& Jia, Baoxiu& Jiao, Chen…[et al.]. Investigation of the Interaction between Patulin and Human Serum Albumin by a Spectroscopic Method, Atomic Force Microscopy, and Molecular Modeling. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-494438
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494438