Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma
المؤلفون المشاركون
Wang, Kui
Xing, Xiang-Lei
Lv, Gang
Li, Jun
Hou, Ying
Shen, Feng
Yang, Xiao-yu
Xia, Yong
Chen, Ke-ji
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-04
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Let-7 family microRNAs have been reported to be downregulated in human hepatocellular carcinoma in comparison with normal hepatic tissues.
Among them, let-7g was identified as the lowest expression using real-time RT-PCR.
However, the mechanism by which let-7g works in hepatocellular carcinoma remains unknown.
Here, in our present study, we have had let-7g reexpressed in vitro in hepatocellular carcinoma cell lines MHCC97-H and HCCLM3 via transfection.
The proliferation after reexpression of let-7g was assayed using MTT method; the migration and invasion after restoration were detected by wound-healing and Transwell assay, respectively.
We found using Western-blotting that let-7g can regulate epithelial-mesenchymal transition (EMT) by downregulating K-Ras and HMGA2A after reexpresssion.
Xenografted nude mice were used to observe whether or not reexpression of let-7g could have potential therapeutic ability.
In vivo, to observe the association with let-7g expression and overall prognosis, 40 paired cases of hepatocellular carcinoma were analyzed using in situ hybridization (ISH).
It was found that reexpression of let-7g can inhibit the proliferation, migration, and invasion significantly, and that low expression of let-7g was significantly associated with poorer overall survival.
Taken together, let-7g could be used as a promising therapeutic agent in vivo in the treatment of hepatocellular carcinoma at the earlier stage.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Ke-ji& Hou, Ying& Wang, Kui& Li, Jun& Xia, Yong& Yang, Xiao-yu…[et al.]. 2014. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-495143
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Ke-ji…[et al.]. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-495143
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Ke-ji& Hou, Ying& Wang, Kui& Li, Jun& Xia, Yong& Yang, Xiao-yu…[et al.]. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-495143
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495143
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر