![](/images/graphics-bg.png)
Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma
Joint Authors
Wang, Kui
Xing, Xiang-Lei
Lv, Gang
Li, Jun
Hou, Ying
Shen, Feng
Yang, Xiao-yu
Xia, Yong
Chen, Ke-ji
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Let-7 family microRNAs have been reported to be downregulated in human hepatocellular carcinoma in comparison with normal hepatic tissues.
Among them, let-7g was identified as the lowest expression using real-time RT-PCR.
However, the mechanism by which let-7g works in hepatocellular carcinoma remains unknown.
Here, in our present study, we have had let-7g reexpressed in vitro in hepatocellular carcinoma cell lines MHCC97-H and HCCLM3 via transfection.
The proliferation after reexpression of let-7g was assayed using MTT method; the migration and invasion after restoration were detected by wound-healing and Transwell assay, respectively.
We found using Western-blotting that let-7g can regulate epithelial-mesenchymal transition (EMT) by downregulating K-Ras and HMGA2A after reexpresssion.
Xenografted nude mice were used to observe whether or not reexpression of let-7g could have potential therapeutic ability.
In vivo, to observe the association with let-7g expression and overall prognosis, 40 paired cases of hepatocellular carcinoma were analyzed using in situ hybridization (ISH).
It was found that reexpression of let-7g can inhibit the proliferation, migration, and invasion significantly, and that low expression of let-7g was significantly associated with poorer overall survival.
Taken together, let-7g could be used as a promising therapeutic agent in vivo in the treatment of hepatocellular carcinoma at the earlier stage.
American Psychological Association (APA)
Chen, Ke-ji& Hou, Ying& Wang, Kui& Li, Jun& Xia, Yong& Yang, Xiao-yu…[et al.]. 2014. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-495143
Modern Language Association (MLA)
Chen, Ke-ji…[et al.]. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-495143
American Medical Association (AMA)
Chen, Ke-ji& Hou, Ying& Wang, Kui& Li, Jun& Xia, Yong& Yang, Xiao-yu…[et al.]. Reexpression of Let-7g MicroRNA Inhibits the Proliferation and Migration via K-RasHMGA2Snail Axis in Hepatocellular Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-495143
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495143