![](/images/graphics-bg.png)
MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization
المؤلفون المشاركون
Yoshinari, Masao
Hayakawa, Tohru
Uo, Motohiro
Yoshida, Eiji
Yoshimura, Yoshitaka
المصدر
International Journal of Biomaterials
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-22
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The present study was aimed to evaluate the viability and total protein contents of osteoblast-like cells on the titanium surface with different surface mechanical treatment, namely, nanometer smoothing (Ra: approximately 2.0 nm) and sandblasting (Ra: approximately 1.0 μm), and biochemical treatment, namely, with or without fibronectin immobilization.
Fibronectin could be easily immobilized by tresyl chloride-activation technique.
MC3T3-E1 cells were seeded on the different titanium surfaces.
Cell viability was determined by MTT assay.
At 1 day of cell culture, there were no significant differences in cell viability among four different titanium surfaces.
At 11 days, sandblasted titanium surface with fibronectin immobilization showed the significantly highest cell viability than other titanium surface.
No significant differences existed for total protein contents among four different titanium surfaces at 11 days of cell culture.
Scanning electron microscopy observation revealed that smoothness of titanium surface produced more spread cell morphologies, but that fibronectin immobilization did not cause any changes of the morphologies of attached cells.
Fibronectin immobilization provided greater amount of the number of attached cells and better arrangement of attached cells.
In conclusion, the combination of sandblasting and fibronectin immobilization enhanced the cell viability and fibronectin immobilization providing better arrangements of attached cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hayakawa, Tohru& Yoshida, Eiji& Yoshimura, Yoshitaka& Uo, Motohiro& Yoshinari, Masao. 2012. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495221
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hayakawa, Tohru…[et al.]. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-495221
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hayakawa, Tohru& Yoshida, Eiji& Yoshimura, Yoshitaka& Uo, Motohiro& Yoshinari, Masao. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495221
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495221
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)