MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization
Joint Authors
Yoshinari, Masao
Hayakawa, Tohru
Uo, Motohiro
Yoshida, Eiji
Yoshimura, Yoshitaka
Source
International Journal of Biomaterials
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The present study was aimed to evaluate the viability and total protein contents of osteoblast-like cells on the titanium surface with different surface mechanical treatment, namely, nanometer smoothing (Ra: approximately 2.0 nm) and sandblasting (Ra: approximately 1.0 μm), and biochemical treatment, namely, with or without fibronectin immobilization.
Fibronectin could be easily immobilized by tresyl chloride-activation technique.
MC3T3-E1 cells were seeded on the different titanium surfaces.
Cell viability was determined by MTT assay.
At 1 day of cell culture, there were no significant differences in cell viability among four different titanium surfaces.
At 11 days, sandblasted titanium surface with fibronectin immobilization showed the significantly highest cell viability than other titanium surface.
No significant differences existed for total protein contents among four different titanium surfaces at 11 days of cell culture.
Scanning electron microscopy observation revealed that smoothness of titanium surface produced more spread cell morphologies, but that fibronectin immobilization did not cause any changes of the morphologies of attached cells.
Fibronectin immobilization provided greater amount of the number of attached cells and better arrangement of attached cells.
In conclusion, the combination of sandblasting and fibronectin immobilization enhanced the cell viability and fibronectin immobilization providing better arrangements of attached cells.
American Psychological Association (APA)
Hayakawa, Tohru& Yoshida, Eiji& Yoshimura, Yoshitaka& Uo, Motohiro& Yoshinari, Masao. 2012. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495221
Modern Language Association (MLA)
Hayakawa, Tohru…[et al.]. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-495221
American Medical Association (AMA)
Hayakawa, Tohru& Yoshida, Eiji& Yoshimura, Yoshitaka& Uo, Motohiro& Yoshinari, Masao. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization. International Journal of Biomaterials. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495221