Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain
المؤلفون المشاركون
Bichenkova, Elena V.
Beloglazova, Natalia G.
Zenkova, Marina A.
Fabani, Martin M.
Sil'nikov, Vladimir V.
Vlassov, Valentin V.
Polushin, Nikolai N.
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-25
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Design of site-selective artificial ribonucleases (aRNases) is one of the most challenging tasks in RNA targeting.
Here, we designed and studied oligonucleotide-based aRNases containing multiple imidazole residues in the catalytic part and systematically varied structure of cleaving constructs.
We demonstrated that the ribonuclease activity of the conjugates is strongly affected by the number of imidazole residues in the catalytic part, the length of a linker between the catalytic imidazole groups of the construct and the oligonucleotide, and the type of anchor group, connecting linker structure and the oligonucleotide.
Molecular modeling of the most active aRNases showed that preferable orientation(s) of cleaving constructs strongly depend on the structure of the anchor group and length of the linker.
The inclusion of deoxyribothymidine anchor group significantly reduced the probability of cleaving groups to locate near the cleavage site, presumably due to a stacking interaction with the neighbouring nucleotide residue.
Altogether the obtained results show that dynamics factors play an important role in site-specific RNA cleavage.
Remarkably high cleavage activity was displayed by the conjugates with the most flexible and extended cleaving construct, which presumably provides a better opportunity for imidazole residues to be correctly positioned in the vicinity of scissile phosphodiester bond.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Beloglazova, Natalia G.& Fabani, Martin M.& Polushin, Nikolai N.& Sil'nikov, Vladimir V.& Vlassov, Valentin V.& Bichenkova, Elena V.…[et al.]. 2011. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-495602
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Beloglazova, Natalia G.…[et al.]. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids No. 2011 (2011), pp.1-17.
https://search.emarefa.net/detail/BIM-495602
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Beloglazova, Natalia G.& Fabani, Martin M.& Polushin, Nikolai N.& Sil'nikov, Vladimir V.& Vlassov, Valentin V.& Bichenkova, Elena V.…[et al.]. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-495602
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495602
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر