Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain
Joint Authors
Bichenkova, Elena V.
Beloglazova, Natalia G.
Zenkova, Marina A.
Fabani, Martin M.
Sil'nikov, Vladimir V.
Vlassov, Valentin V.
Polushin, Nikolai N.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-25
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Design of site-selective artificial ribonucleases (aRNases) is one of the most challenging tasks in RNA targeting.
Here, we designed and studied oligonucleotide-based aRNases containing multiple imidazole residues in the catalytic part and systematically varied structure of cleaving constructs.
We demonstrated that the ribonuclease activity of the conjugates is strongly affected by the number of imidazole residues in the catalytic part, the length of a linker between the catalytic imidazole groups of the construct and the oligonucleotide, and the type of anchor group, connecting linker structure and the oligonucleotide.
Molecular modeling of the most active aRNases showed that preferable orientation(s) of cleaving constructs strongly depend on the structure of the anchor group and length of the linker.
The inclusion of deoxyribothymidine anchor group significantly reduced the probability of cleaving groups to locate near the cleavage site, presumably due to a stacking interaction with the neighbouring nucleotide residue.
Altogether the obtained results show that dynamics factors play an important role in site-specific RNA cleavage.
Remarkably high cleavage activity was displayed by the conjugates with the most flexible and extended cleaving construct, which presumably provides a better opportunity for imidazole residues to be correctly positioned in the vicinity of scissile phosphodiester bond.
American Psychological Association (APA)
Beloglazova, Natalia G.& Fabani, Martin M.& Polushin, Nikolai N.& Sil'nikov, Vladimir V.& Vlassov, Valentin V.& Bichenkova, Elena V.…[et al.]. 2011. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-495602
Modern Language Association (MLA)
Beloglazova, Natalia G.…[et al.]. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids No. 2011 (2011), pp.1-17.
https://search.emarefa.net/detail/BIM-495602
American Medical Association (AMA)
Beloglazova, Natalia G.& Fabani, Martin M.& Polushin, Nikolai N.& Sil'nikov, Vladimir V.& Vlassov, Valentin V.& Bichenkova, Elena V.…[et al.]. Site-Selective Artificial Ribonucleases : Oligonucleotide Conjugates Containing Multiple Imidazole Residues in the Catalytic Domain. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-17.
https://search.emarefa.net/detail/BIM-495602
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495602