Xuezhikang Therapy Increases miR-33 Expression in Patients with Low HDL-C Levels

الملخص EN

Background.

MicroRNA-33a and -b (miR-33a/b) have been revealed to be posttranscriptional regulators of HDL metabolism.

Xuezhikang (XZK) is a marked natural HDL-raising polypill.

We aim to evaluate the effects of XZK on the expression of circulating miR-33a/b in patients with low plasma HDL-C levels.

Methods.

A total of 42 participating patients with low baseline levels of HDL cholesterol were assigned to receive an XZK capsule, 600 mg twice daily for 6 months.

The expression of circulating miR-33a/b was detected at baseline and after XZK therapy measured with quantitative reverse-transcription (RT) polymerase chain reaction (PCR).

Results.

The mean (SD) HDL-C level after XZK treatment was 1.19 (0.13) mmol/L, representing an increase of 11.2% from baseline (P<0.001).

Q-PCR analysis of plasma miRNAs revealed an increase in relative miR-33a/b expression with XZK treatment.

The miR-33a expression was raised from 0.81 to 1.73 (P=0.012); miR-33b expression was increased from 1.2 to 2.75 (P<0.001).

The changes of miR-33a and miR-33b were inversely related to the posttreatment LDL-C levels (r=-0.37, P=0.019; r=-0.33, P=0.035, resp.).

Conclusion.

In patients with low HDL-C levels, XZK therapy raised plasma levels of miR-33a and miR-33b, which may inhibit cellular cholesterol export and limit the HDL-raising effect of XZK.