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Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation
المؤلفون المشاركون
Levi, Renzo
Ramella, Roberta
Tota, Bruno
Mahata, Sushil K.
Fornero, Sara
Gallina, Clara
Gallo, Maria Pia
Alloatti, Giuseppe
Bassino, Eleonora
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-20
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The chromogranin-A peptide catestatin modulates a wide range of processes, such as cardiovascular functions, innate immunity, inflammation, and metabolism.
We recently found that the cardiac antiadrenergic action of catestatin requires a PI3K-dependent NO release from endothelial cells, although the receptor involved is yet to be identified.
In the present work, based on the cationic properties of catestatin, we tested the hypothesis of its interaction with membrane heparan sulphate proteoglycans, resulting in the activation of a caveolae-dependent endocytosis.
Experiments were performed on bovine aortic endothelial cells.
Endocytotic vesicles trafficking was quantified by confocal microscopy using a water-soluble membrane dye; catestatin colocalization with heparan sulphate proteoglycans and caveolin 1 internalization were studied by fluorimetric measurements in live cells.
Modulation of the catestatin-dependent eNOS activation was assessed by immunofluorescence and immunoblot analysis.
Our results demonstrate that catestatin (5 nM) colocalizes with heparan sulphate proteoglycans and induces a remarkable increase in the caveolae-dependent endocytosis and caveolin 1 internalization, which were significantly reduced by both heparinase and wortmannin.
Moreover, catestatin was unable to induce Ser1179 eNOS phosphorylation after pretreatments with heparinase and methyl-β-cyclodextrin.
Taken together, these results highlight the obligatory role for proteoglycans and caveolae internalization in the catestatin-dependent eNOS activation in endothelial cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fornero, Sara& Bassino, Eleonora& Ramella, Roberta& Gallina, Clara& Mahata, Sushil K.& Tota, Bruno…[et al.]. 2014. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-497740
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fornero, Sara…[et al.]. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-497740
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fornero, Sara& Bassino, Eleonora& Ramella, Roberta& Gallina, Clara& Mahata, Sushil K.& Tota, Bruno…[et al.]. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-497740
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497740
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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