Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation
Joint Authors
Levi, Renzo
Ramella, Roberta
Tota, Bruno
Mahata, Sushil K.
Fornero, Sara
Gallina, Clara
Gallo, Maria Pia
Alloatti, Giuseppe
Bassino, Eleonora
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The chromogranin-A peptide catestatin modulates a wide range of processes, such as cardiovascular functions, innate immunity, inflammation, and metabolism.
We recently found that the cardiac antiadrenergic action of catestatin requires a PI3K-dependent NO release from endothelial cells, although the receptor involved is yet to be identified.
In the present work, based on the cationic properties of catestatin, we tested the hypothesis of its interaction with membrane heparan sulphate proteoglycans, resulting in the activation of a caveolae-dependent endocytosis.
Experiments were performed on bovine aortic endothelial cells.
Endocytotic vesicles trafficking was quantified by confocal microscopy using a water-soluble membrane dye; catestatin colocalization with heparan sulphate proteoglycans and caveolin 1 internalization were studied by fluorimetric measurements in live cells.
Modulation of the catestatin-dependent eNOS activation was assessed by immunofluorescence and immunoblot analysis.
Our results demonstrate that catestatin (5 nM) colocalizes with heparan sulphate proteoglycans and induces a remarkable increase in the caveolae-dependent endocytosis and caveolin 1 internalization, which were significantly reduced by both heparinase and wortmannin.
Moreover, catestatin was unable to induce Ser1179 eNOS phosphorylation after pretreatments with heparinase and methyl-β-cyclodextrin.
Taken together, these results highlight the obligatory role for proteoglycans and caveolae internalization in the catestatin-dependent eNOS activation in endothelial cells.
American Psychological Association (APA)
Fornero, Sara& Bassino, Eleonora& Ramella, Roberta& Gallina, Clara& Mahata, Sushil K.& Tota, Bruno…[et al.]. 2014. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-497740
Modern Language Association (MLA)
Fornero, Sara…[et al.]. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-497740
American Medical Association (AMA)
Fornero, Sara& Bassino, Eleonora& Ramella, Roberta& Gallina, Clara& Mahata, Sushil K.& Tota, Bruno…[et al.]. Obligatory Role for Endothelial Heparan Sulphate Proteoglycans and Caveolae Internalization in Catestatin-Dependent eNOS Activation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-497740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497740